Variations of SPARC in obese patients with type 2 diabetes mellitus

VernacularTitle:2型糖尿病肥胖患者SPARC表达差异性研究
Author: Kai LIU ; Haiyan SONG ; Shu LIU ; Lei LI ; Kun LI ; Xiaoyao YANG
Publication Type:Journal Article
Keywords: Secreted protein acidic and rich in cysteine; Obesity; Diabetes mellitus,type 2
From: Chinese Journal of Endocrinology and Metabolism 2014;30(5):388-392
CountryChina
Language:Chinese
Abstract: Objective To observe serum secreted protein acidic and rich in cysteine (SPARC) levels in normal subjects,obese subjects,patients with type 2 diabetes mellitus (T2DM),and obese patients with T2DM,as well as the difference of SPARC expression in mesenteric adipose tissue of subjects with and without T2DM.Methods Serum SPARC level was measured with the ELISA.RT-PCR,Western blot,and immunofluorescence were used to examine SPARC mRNA and protein expressions in mesenteric adipose tissue.Results (1) SPARC levels were higher in obesity,T2DM,and T2DM with obesity groups compared with normal group [(1 191.6 ± 718.91,1 223.81 ± 645.96,1 538.01 ± 757.95 vs 851.07 ± 280.21) ng/L,P＜0.05].(2) Pearson correlation analysis showed that SPARC level was positively correlated with homeostasis model assessment for insulin resistance index (r =0.205,P＜ 0.05).(3) The expression levels of SPARC mRNA and protein in mesenteric adipose tissue of T2DM patients were higher than those of control subjects (P ＜ 0.05).Conclusion SPARC is closely related to the development of obesity,insulin resistance,and type 2 diabetes mellitus.