Protective effect of trichostatin a and 5-azacitidine on cytokine-induced toxicity in pancreatic β-cells
10.3760/cma.j.issn.1000-6699.2014.04.011
- VernacularTitle:曲古霉素A和5-氮杂胞苷对细胞因子诱导损伤的胰岛β细胞的保护作用
- Author:
Can HOU
;
Yanfei WANG
;
Yi LIN
;
Gongping LIANG
;
Qianjin LU
;
Zhiguang ZHOU
- Publication Type:Journal Article
- Keywords:
Pancreatic β-cells;
Interleukin-1 β;
Interferon-γ;
Trichostatin A;
5-azacitidine
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(4):321-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of trichostatin A (TSA) and 5-azacitidine (5-AzaC) on pancreatic β-cells impaired by cytokine,via measuring the proliferation,apoptosis,and function of pancreatic β-cells.Methods RIN-m5f was impaired by interleukin-1β and interferon-γin vitro,and treated with TSA and 5-AzaC.Experiment groups included blank control group,cytokine induction group,0.05/0.10 μmoL/L TSA group,0.63/1.25 μmoL/L 5-AzaC group,and0.10 μmol/L TSA plus 1.25 μmol/L 5-AzaC group.The viability of RIN-m5f cells was detected by MTT assay.Apoptotic rate was determined by Annexin V-fluorescein isothiocyanate (FITC) /propidium iodide flow cytometry.Insulin secretion was measured by enzyme-linked immunosorbent assay.Results The viability of RIN-m5f cells in 0.05/0.10 μmoL/L TSA group,0.63/1.25 μmol/L 5-AzaC group,and 5-AzaC plus TSA group was 70.1％/79.2 ％,67.3 ％/82.9 ％,and 89.1％ respectively,being higher than that in the cytokine group (33.9％,P＜0.05) ; the apoptosis rate was 10.3％/10.5％,7.9％/9.6％,and 8.2％,being lower than that in the cytokine group (16.6％,P＜0.05) ; the capacity of glucose-stimulated insulin secretion of all the treated groups was higher than that in the cytokine group (P＜0.05).Conclusion TSA and 5-AzaC might promote the proliferation of pancreatic β-cells impaired by cytokines,inhibit its apoptosis and recover its insulin secretion.
