Effects of ulinastatin pretreatment on cognitive dysfunction induced by chronic exposure to ketamine in immature mice
10.3760/cma.j.issn.0254-1416.2014.02.005
- VernacularTitle:乌司他丁预先给药对氯胺酮慢性暴露致幼鼠认知功能障碍的影响
- Author:
Yu HONG
;
Shouping WANG
;
Shuling PENG
;
Ting LIU
;
Yingzhen CHEN
;
Lisheng ZHOU
;
Libing ZHOU
- Publication Type:Journal Article
- Keywords:
Trypsin inhibitors;
Ketamine;
Cognition disorders;
Adolescent
- From:
Chinese Journal of Anesthesiology
2014;34(2):143-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of ulinastatin pretreatment on cognitive dysfunction induced by chronic exposure to ketamine in immature mice.Methods Thirty-six healthy male C57BL/6 mice,aged 21 days,weighing 20-30 g,were randomly divided into 3 groups (n =12 each) using a random number table:control group (group C),ketamine group (group K),and ulinastatin pretreatment group (group U).In K and U groups,ketamine 30 mg/kg was injected intraperitoneally three times a day at 30-minute intervals for 21 consecutive days,while in group U,ulinastatin 50 000 U/kg was injected intraperitoneally at 30 min before the first injection of ketamine everyday.Cognitive function was assessed using Morris water maze and open field tests at 24 h after the last administration of ketamine.Mice in each group were sacrificed immediately after the end of the tests and hippocampi were harvested to determine the contents of interleukin-6 (IL-6),IL-1 and tumor necrosis factor-α (TNF-α) using ELISA.Results Compared with group C,the escape latency was significantly prolonged,the time spent in the original platform and in the central area for the open field was shortened,the frequency of crossing the original platform was decreased,and the contents of IL-1,IL-6,and TNF-α were increased in group K (P ＜ 0.05),while there were no significant differences in the indexes mentioned above in group U (P ＞ 0.05).Compared with group K,the escape latency was significantly shortened,the time spent in the original platform and in the central area for the open field was prolonged,the frequency of crossing the original platform was increased,and the contents of IL-1,IL-6,and TNF-α were decreased in group U (P ＜ 0.05).Conclusion Ulinastatin pretreatment can improve cognitive dysfunction induced by chronic exposure to ketamine in immature mice,and inhibition of inflammatory responses in hippocampi may be involved in the mechanism.
