Effect of sevoflurane pretreatment on inflammatory response during renal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2014.01.033
- VernacularTitle:七氟醚预先给药对大鼠肾缺血再灌注时炎性反应的影响
- Author:
Jing WANG
;
Ling LIU
;
Lin ZHANG
;
Lirong GONG
;
Hui YUE
;
Quanyong YANG
;
Jianbo YU
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Reperfusion injury;
Kidney;
Inflammation
- From:
Chinese Journal of Anesthesiology
2014;34(1):120-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane pretreatment on the inflammatory response during renal ischemia-reperfusion (I/R) in rats.Methods Thirty pathogen-free male Sprague-Dawley rats,aged 12-14 weeks,weighing 220-260 g,were randomized into 3 groups (n =10 each) using a random number table:sham operation group (group S),I/R group and sevoflurane pretreatment group (group SP).Renal I/R was induced by clamping the left renal pedicle for 45 min followed by reperfusion in I/R and SP groups.In group S inhalation of 3％ sevoflurane in O2 was started at 30 min before I/R and maintained throughout the experiment.Venous blood samples were taken at 3 h of reperfusion for determination of serum blood urea nitrogen (BUN) and creatinine (Cr) concentrations.The animals were then sacrificed and the left kidneys were removed for microscopic examination and for measurement of the content of tumor necrosis factor-apha (TNF-α),interleukin-6 (IL-6) and intercellular cell adhesion molecule-1 (ICAM-1) in renal tissues (by ELISA).Results Compared with group S,the serum BUN and Cr concentrations,severity of necrosis of renal proximal convoluted tubules (0 =normal,4 =necrosis of whole segment of proximal convoluted tubules),and contents of TNF-α,IL-6 and ICAM-1 were significantly increased in I/R and SP groups (P ＜ 0.05).Compared with group I/R,the serum BUN and Cr concentrations,severity of necrosis of renal proximal convoluted tubules,and contents of TNF-α,IL-6 and ICAM-1 were significantly decreased in SP group (P ＜ 0.05).Conclusion Sevoflurane pretreatment can protect kidney against I/R injury by inhibiting the inflammatory responses in the renal tissues of rats.
