Effect of sevoflurane anesthesia on activation of gliocytes in aged rat hippocampi
10.3760/cma.j.issn.0254-1416.2014.02.006
- VernacularTitle:七氟醚麻醉对老龄大鼠海马胶质细胞活化的影响
- Author:
Meihua YANG
;
Miaomiao WANG
;
Nan HU
;
Chao WANG
;
Yize LI
;
Haiyun WANG
;
Guolin WANG
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Microglia;
Astrocytes;
Aged;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2014;34(2):147-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane anesthesia on activation of gliocytes in aged rat hippocampi.Methods One hundred and four male Wistar rats,weighing 600-650 g,were randomly divided into 2 groups (n =52 each) using a random number table:control group (group C) and sevoflurane group (group S).Group S inhaled 3.6％ sevoflurane for 2 h,and open reduction and internal fixation was performed after tibial fracture was induced.Y-maze test and fear conditioning test were carried out on days 1,3 and 7 after surgery.Six rats of each group were chosen on days 1,3 and 7 after surgery and hippocampi were removed to detect the activation of microglias and astrocytes (by immunofluorescence) and expression of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) (by Western blot).Results Compared with group C,the number of entries into each arm was significantly reduced,the duration of N arm stay was shortened,the percentage of time spent freezing induced by condition and the percentage of time spent freezing induced by context were decreased,and the activation of microglias and astrocytes was increased,and the expression of IL-6 and TNF-α was up-regulated in S groups (P ＜ 0.05).Conclusion Sevoflurane-induced postoperative cognitive dysfunction may be related to enhanced activation of gliocytes in aged rat hippocampi.
