Effects of curcumin preconditioning on activity of xanthine oxidase during intestinal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2014.01.028
- VernacularTitle:姜黄素预处理对大鼠肠缺血再灌注时黄嘌呤氧化酶活性的影响
- Author:
Meijuan LIAO
;
Weicheng ZHAO
;
Xueqin ZHENG
;
Jian HE
;
Chengxiang YANG
;
Hanbing WANG
;
Hongzhen LIU
- Publication Type:Journal Article
- Keywords:
Curcumin;
Reperfusion injury;
Intestines;
Xanthine oxidase
- From:
Chinese Journal of Anesthesiology
2014;34(1):102-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of curcumin preconditioning on the activity of xanthine oxidase (XOD) during intestinal ischemia-reperfusion (I/R) in rats.Methods Thirty female Sprague-Dawley rats,aged 180-220 g,were randomly divided into 3 groups (n =10 each) using a random number table:sham operation group (S group),intestinal I/R group (I/R group),and curcumin preconditioning group (Cur group).Intestinal I/R was induced by clamping the superior mesenteric artery for 75 min followed by reperfusion.Curcumin 200 mg/kg was given everyday for 5 days before intestinal I/R in Cur group and the equal volume of normal saline was given instead of curcumin in S and I/R groups.The rats were sacrificed at 4 h of reperfusion and the intestinal specimens were obtained for microscopic examination of pathologic changes which were graded using Chiu scoring system and for determination of XOD activity,content of malondialdehyde (MDA),and superoxide dismutase (SOD) activity.Results Compared with S group,the Chiu score,activity of XOD and content of MDA were significantly increased,while the activity of SOD was decreased in I/R and Cur groups (P ＜ 0.05).Compared with I/R group,the Chiu score,activity of XOD and content of MDA were significantly decreased,and the activity of SOD was increased in Cur group (P ＜ 0.05).Conclusion Curcumin preconditioning can attenuate intestinal I/R injury in rats,which may be due to inhibition of XOD activity and decreased oxidative stress in intestinal tissues.
