Clinical analysis of the inconsistency of antineutrophil cytoplasmic antibodies test results between indirect immune fluorescence and enzyme-linked immunosorbent assay in 589 cases
10.3760/cma.j.issn.1007-7480.2014.04.011
- VernacularTitle:联合使用间接免疫荧光法和酶联免疫吸附法检测抗中性粒细胞胞质抗体结果不一致589例临床分析
- Author:
Zhijie ZENG
;
Yanhong SUN
;
Liqin SUN
;
Haiyan HUANG
;
Tang JIANG
- Publication Type:Journal Article
- Keywords:
Antibodies,antineutrophil cytoplasmic;
Fluorescent antibody technique,inderect;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Rheumatology
2014;18(4):263-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the clinical significance and features of patient s' with inconsistent antineutrophil cytoplasmic antibodies (ANCA) test results between indirect immune fluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA).Methods ANCA were detected with IIF and ELISA method jointly among 12 386 in-patients and a retrospective analysis on the proportion of clinical features and significance was made in 589 cases with inconsistent results using Microsoft Excel 2007 statistical software.Results Among the 589 patients,68 (11.5％) were diagnosed as vasculitis,in which 51 cases as ANCA-associated vasculitis,and 521(88.4％) were diagnosed as non-vasculitis including 181 connective disease and 340 non-connective diseases in which hypertension and cardiopathy were common.The common inconsistent results of ANCA were p-ANCA/ELISA (-),IIF (-)/anti-MPO (+),IIF (-)/anti-PR3 (+),IIF (-)/anti-PR3 (+) anti-MPO (+) accounted for 24.4％ (144/589),29.5％ (174/589),15.9％ (94/589),18.5％ (108/589) respectively,these accounted for 88.3％(520/589) of total inconsistency.Conclusion The spectrum of diseases and clinical characteristics varies widely and often presents with multiorgan involvement in patients with inconsistent ANCA results.These reasons make it easy to be misdiagnosed.Attention should be paid to identify and different these inconsistency.
