Dose-effect of nucleoplasmic bridges frequencies in human peripheral blood lymphocytes induced by 60Co γ-rays
10.3760/cma.j.issn.0254-5098.2014.04.005
- VernacularTitle:60Co γ射线诱导人外周血淋巴细胞核质桥的剂量-效应关系研究
- Author:
Hua ZHAO
;
Xue LU
;
Deqing CHEN
;
Qingjie LIU
- Publication Type:Journal Article
- Keywords:
Ionizing radiation;
Nucleoplasmic bridge;
Micronucleus;
Dose response
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(4):259-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the analysis criteria of nucleoplasmic bridges (NPB) and a dose-response curve of NPB frequencies in human peripheral blood lymphocytes irradiated by 60Co γ-rays.Methods Human peripheral blood samples were collected from three healthy males,and were irradiated with 0,1,2,3,4,5 and 6 Gy of 60Co γ-rays.A cytokinesis-block micronucleus assay was carried out to analyze NPBs and micronuclei (MN) in binucleated cells.Results NPBs in binucleated cells at each dose level of γ-ray was conformed to the Poisson distribution.The dose-response curve of the γ-ray-induced NPB frequencies in human peripheral lymphocytes followed the linear-quadratic model y =(1.39 × 10-3)x2 + (4.94 × 10-3)x (R2 =0.981,P ＜ 0.05).Conclusions The dose-response curve of NPB frequencies in human peripheral blood lymphocytes induced by 0-6 Gy 60Co γ-rays was established.
