Preosteoblastic differentiation of MC3T3 E1 cells on the new-type self-assembling peptide hydrogel NBD/RADA16
10.3969/j.issn.2095-4344.2014.21.009
- VernacularTitle:新型自组装水凝胶NBD/RADA16可促进前成骨细胞的分化
- Author:
Gang ZHAO
;
Jingling LI
;
Dan WANG
;
Jing YU
;
Yanyu ZHAO
;
Dapeng MAN
- Publication Type:Journal Article
- Keywords:
hydrogel;
peptides;
osteoblasts
- From:
Chinese Journal of Tissue Engineering Research
2014;(21):3329-3333
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:RADA16 is a mature amphiphilic self-assembling peptide, which can be assembled into nanofibers, and promote MC3T3 E1 cellattachment, spreading and proliferation.
OBJECTIVE:To observe the effects of the new-type self-assembling peptide hydrogel NBD/RADA16 on osteogenic differentiation of mouse preosteoblasts MC3T3 E1.
METHODS:The MC3T3 E1 cells were inoculated in NBD/RADA16 self-assembling peptide hydrogel and RADA16 hydrogel for osteogenic induction. cells undergoing simple osteogenic induction served as controls. After culture for 1, 3, 6 days, the activity of alkaline phosphatase was detected. After 7 days, western blot assay was used to determine the expression of bone morphogenetic protein-2. After 21 days, alizarin red staining was used to observe calcified nodules.
RESULTS AND CONCLUSION:MC3T3 E1 cells grew wel on the NBD/RADA16 peptide hydrogel, which were superior to those on the RADA16 hydrogel. The activity of alkaline phoshpatase was higher in the NBD/RADA16 group than the RADA16 and control groups (P<0.01). Compared with the RADA16 hydrogel, mineralized matrix deposition and expression of bone morphogenetic protein-2 were higher on the NBD/RADA16 peptide hydrogel (P<0.01). These findings indicate that the NBD/RADA16 peptide hydrogel is superior to the RADA16 hydrogel for promoting the osteogenic differentiation of MC3T3 E1 cells.
