Expression of transient receptor potential M8 in human knee joint cartilage
10.3969/j.issn.2095-4344.2014.20.001
- VernacularTitle:瞬时感受电位M8在人类膝关节软骨组织中的表达
- Author:
Bin YU
;
Boyuan NIE
;
Zhaohui YANG
- Publication Type:Journal Article
- Keywords:
transient receptor potential channels;
cartilage;
knee joint;
blotting,western;
immunofluorescence
- From:
Chinese Journal of Tissue Engineering Research
2014;(20):3117-3121
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Transient receptor potential protein channel is distributed in many kinds of tissue and cells in human body, and takes part in many physiologic functions and pathological changes of human body. However, up to now, transient receptor potential melastatin 8 (TRPM8) has not been reported in human cartilage.
OBJECTIVE:To study the expression of TRPM8, a subfamily member of transient receptor potential gene, in human knee joint cartilage.
METHODS:Fifteen samples were selected from patients after amputatioin due to severe trauma. Total protein was extracted from 300 mg cartilage tissue of every patient. To detect the distribution of TRPM8 in human
cartilage, cartilage sections were col ected in the same region. The expression and distribution of TRPM8 in human cartilage was detected by western blot analysis and immunofluorescence respectively.
RESULTS AND CONCLUSION:Western blot analysis showed that specific TRPM8 band was visible at molecular weight of 130 000, and as the age increased, TRPM8 expression in human cartilage was gradual y decreased. Immunofluorescence staining results showed that TRPM8 was evenly located in the cellmembrane of chondrocytes. TRPM8 is expressed in the human knee cartilage, specifical y in member of chondrocytes.
