Influence of geniposide on collagen II synthesis of cultured chondrocytes in vitro
10.3969/j.issn.2095-4344.2014.20.007
- VernacularTitle:京尼平苷干预体外培养大鼠软骨细胞Ⅱ型胶原蛋白的合成
- Author:
Yulin TAN
;
Tongzhu BAO
;
Qin LIU
;
Yu HAN
;
Yanlin WANG
;
Yang LIU
;
Naxin ZHOU
;
Fei YAN
;
Longtao ZHAO
- Publication Type:Journal Article
- Keywords:
chondrocytes;
arthritis;
col agen;
cellular structure;
articular cartilage
- From:
Chinese Journal of Tissue Engineering Research
2014;(20):3149-3152
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Chinese herb Cape Jasmine Fruit can activate the chondrocytes and geniposide is an important component in this herb.
OBJECTIVE:To investigate the effect of geniposide on col agen II synthesis in rat chondrocytes cultured in vitro. METHODS:Rat chondrocytes were separated and cultured in vitro. The chondrocytes were then interfered with 25, 50 and 100 mmol/L geniposide. Normal control group was also set. Col agen II mRNA and protein expression was detected with semi-quantitative RT-PCR and western blot analysis, respectively.
RESULTS AND CONCLUSION:RT-PCR and western blot analysis results showed that, geniposide at 25, 50 and 100 mmol/L increased the col agen II mRNA and protein expression (P<0.01). Geniposide can promote the synthesis of col agen II in rat chondrocytes cultured in vitro.
