Effect of basic fibroblast growth factor on human periodontal ligament cells cultured in vitro
10.3969/j.issn.2095-4344.2014.20.013
- VernacularTitle:碱性成纤维细胞生长因子对体外培养人牙周膜细胞的作用
- Author:
Yangdong LIN
;
Bo ZHAO
;
Kai YIN
- Publication Type:Journal Article
- Keywords:
periodontal ligament;
cells;
fibroblast growth factor 2;
cellproliferation
- From:
Chinese Journal of Tissue Engineering Research
2014;(20):3184-3189
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells.
OBJECTIVE:To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro.
METHODS:Human periodontal ligament cells at passage 5 were inoculated into the 96-wel plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured inα-MEM containing 15%fetal bovine serum and 0, 1, 10, 100μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cellproliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days.
RESULTS AND CONCLUSION:There were significant differences in the proliferation of human periodontal ligament cells among the four groups (F=6.586, P=0.024). As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradual y increased and reached the peak in 100μg/L basic fibroblast growth factor group (P<0.05). The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group (P=0.000), the higher the concentration was, the lower activity was (P<0.05). Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.
