Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells
10.3969/j.issn.2095-4344.2014.20.019
- VernacularTitle:不同浓度人羊膜匀浆上清液培养大鼠许旺细胞的增殖
- Author:
Liang LIU
;
Lei WANG
;
Yalin TONG
;
Yongliang MO
;
Lu LV
;
Yunpeng CHEN
;
Wenxian YANG
;
Lifang LV
;
Qiu ZHAN
;
Fujun ZHU
;
Haiming XIN
;
Zhenyu GONG
- Publication Type:Journal Article
- Keywords:
amnion;
Schwann cells;
cellproliferation;
cytokines
- From:
Chinese Journal of Tissue Engineering Research
2014;(20):3218-3222
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Schwann cells are important celllines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells.
OBJECTIVE:To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell96.
METHODS:Schwann cell96 was cultured with high-glucose DMEM containing 20%fetal bovine serum, and the second generation of Schwann cell96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium.
RESULTS AND CONCLUSION:The total protein concentration of human amniotic homogenate supernatant was 675μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cellproliferation rate of the 10%and 15%concentration groups was greater than that in 20%and 25%concentration groups (P<0.05);10%and 15%concentrations promoted cellproliferation, while 20%and 25%concentrations inhibited cellproliferation. There were no significant difference in the viability of Schwann cell96 between the control group and the experimental group (P>0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cellproliferation.
