A study on establishment of peptide mapping database of Candida albicans
10.3969/j.issn.1673-4130.2014.10.004
- VernacularTitle:建立白色念珠菌蛋白指纹库的研究
- Author:
Yongchang YANG
;
Hua YU
;
Hua LIU
;
Daiwen XIAO
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
peptide mapping;
spectrometry,mass,matrix-assisted laser desorption-ionization
- From:
International Journal of Laboratory Medicine
2014;(10):1240-1242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the establishment of peptide mapping database of Candida albicans ,laying the foundation for rapid diagnosis of Candida albicans infection .Methods 96 Candida albicans were collected clinically ,and its DNA was extracted . Polymerase chain reaction(PCR) was used to amplify the ITS1-5 .8S-ITS2 gene fragments and restriction endonucleases were a-dopted to identify them .Surface enhanced laser desorption ionization-time of flight-mass spectrometry(SELDI-TOF-MS) instrument was applied to detect the Candida albicans peptide mapping ,and Ciphergen ProteinChip software was used to collect data automati-cally .The established peptide mapping database was verified by confirmed Candida .Results According to restriction fragment length polymorphism analysis ,96 strains were confirmed as Candida albicans .15 peptide peaks were captured by SELDI-TOF-MS chips .Five peptide peaks of them with stable expression were screened out ,and the similarity analysis software was used to estab-lish peptide mapping database of Candida albicans .More than 95% of similarity was found between peptide mapping of Candida albicans and established database ,while less than 50% was found between peptide mapping of other Candida species and database . Conclusion The establishment of peptide mapping database of Candida albicans provides a theoretical basis for the rapid diagnosis of Candida albicans infection .
