Identification and virulence gene detection of non-O1 and non-O139 Vibrio chol-erae isolates causing septicemia
- VernacularTitle:致血流感染非 O1群非 O139群霍乱弧菌的鉴定及毒力基因检测
- Author:
Jiuming ZOU
;
Aiping ZHANG
;
Zhishan LI
;
Yan YANG
;
Jianzhong ZHAO
- Publication Type:Journal Article
- Keywords:
Vibrio cholerae;
virulence;
gene;
serotyping
- From:
Chinese Journal of Infection and Chemotherapy
2014;(3):186-189
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify an suspected Vibrio cholerae isolated from Xiangyang Central Hospital and characterize the strain in terms of antibiotic resistance and relevant virulence genes.Methods Pathogen identification and susceptibility testing were completed with MicroScan WalkAway 40 Automated Microbiology System.Slide agglutination was used for serotyping. PCR and sequencing technology were employed for 16s RNA gene analysis.PCR technique was used to detect six major viru-lence genes.Results This suspectedVibrio cholerae isolate was confirmed as non-O1 and non-O139 Vibrio cholerae .Suscep-tibility testing results indicated that the strain was sensitive to ampicillin,chloramphenicol,trimethoprim-sulfamethoxazole, and tetracycline.16s RNA gene sequence analysis showed 100% homologous with the registered sequence in National Center for Biotechnology Information database.Virulence genes rtxC and toxR were identified.The other virulence genes such as tcpAET,ctxA,hlyA,and tcpACL were negative.Conclusions This suspected Vibrio cholerae isolate is confirmed as non-O1 and non-O139 Vibrio cholerae .The pathogenic factors may be related to the virulence genes rtxC and toxR.