Physicochemical properties of chitosan microspheres/silk fibroin/calcium sulfate bone cement
10.3969/j.issn.2095-4344.2014.12.005
- VernacularTitle:壳聚糖微球复合丝素基硫酸钙骨水泥的理化特性
- Author:
Peng WANG
;
Bin PI
;
Jinning WANG
;
Xuesong ZHU
;
Huilin YANG
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
calcium sulfate;
chitosan;
silk
- From:
Chinese Journal of Tissue Engineering Research
2014;(12):1831-1838
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Calcium sulfate used in kyphoplasty and vertebrolplasty has good physical and chemical properties, exerts no toxic effects on human body and has the degradation performance. But its main drawback is rapid degradation.
OBJECTIVE:To develop a chitosan microsphere with silk fibroin/calcium sulfate cement to prepare drug carrier system.
METHODS:Chitosan microspheres were prepared by the emulsion method. Scanning electron microscopy, particle size analysis and swel ing rate were used to study the properties of the microspheres. Different silk concentrations (3%, 6%and 9%) and weight rates (0.5%,1%and 5%) of chitosan microspheres were used to determine the best formula which has the strongest mechanical properties. The composition of this composite bone cement was detected by using X-ray diffraction and Fourier transform infrared spectroscopy.
RESULTS AND CONCLUSION:When the concentration of silk fibroin was 6%and weight rate of chitosan microspheres was 0.5%, we could obtain the maximum compressive strength, which was (39.17±1.96) MPa. With this composition, the initial setting time was (12.99±1.63) minutes and the final setting time was (21.55±0.54) minutes. The results from X-ray diffraction and Fourier transform infrared spectroscopy demonstrated that the main phase composition was calcium sulfate, and silk and chitosan were also included. The composite chitosan microspheres exhibited a slightly wrinkled surface, but were stil intact in spherical shape, indicating the preparation of chitosan microspheres/silk fibroin/calcium sulfate cement was reliable and the product had good structures and properties.
