Application and characteristics of self-assembling peptide nanofiber hydrogel in three-dimensional cell culture systems
10.3969/j.issn.2095-4344.2014.12.017
- VernacularTitle:自组装多肽纳米纤维水凝胶在细胞三维培养中的应用及特征
- Author:
Xi LIU
;
Xiumei WANG
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
peptides;
hydrogel;
cel s;
nanofibers
- From:
Chinese Journal of Tissue Engineering Research
2014;(12):1907-1913
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties.
OBJECTIVE:To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cel culture in cel therapy applications.
METHODS:Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were“self-assembly peptide, tissue engineering”in English and Chinese, respectively. Final y 48 of 224 articles about the design, fictionalization, and three-dimensional cel culture of peptide scaffolds were included in this review.
RESULTS AND CONCLUSION:Self-assembling peptide could undergo spontaneous assembly into wel-ordered interwoven nanofibers in water and rapidly form hydrogel, which physical y mimics the architecture of natural extracel ular matrix to ensure a real three-dimensional microenvironment for cel s. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cel adhesion, proliferation, and differentiation.
