Role of caspase-3 in apoptosis of human umbilical vein endothelial cell induced by heat stress
10.3969/j.issn.1006-5725.2014.06.012
- VernacularTitle:Caspase-3在热打击诱导人脐静脉内皮细胞凋亡中的作用
- Author:
Li LI
;
Zhengtao GU
;
Zhifeng LIU
;
Lei SU
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cell;
Apoptosis;
Caspase-3;
Heat stress
- From:
The Journal of Practical Medicine
2014;(6):871-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of activation of caspase-3 on apoptosis of human umbilical vein endothelial cells induced by heat stress. Methods A model of human umbilical vein endothelial cell heat stress was established. The endothelial cells in the control group were incubated at 37 ℃, while the cells in the heat stress group were incubated at 39 ℃, 41 ℃, or 43 ℃ for 2 h , then all the cells were further incubated at 37 ℃ for 24 h. Apoptosis was detected using staining with Hoechst33258; protein levels of caspase-3 were analyzed by Western blot; and the effect of caspase-3 inhibitor Z-DEVD-FMK on heat stress-induced apoptosis was determined. Results As compared with the control group, with an elevation in heat stress temperature (41℃ to 43 ℃), apoptosis was markedly increased and level of caspase-3 was significantly increased. In addition , caspase-3 inhibitor Z-DEVD-FMK significantly decreased the heat stress-induced apoptosis and levels of caspase-3. Conclusions Caspase-3 mediates apoptosis of human umbilical vein endothelial cells can be induced by heat stress.
