Influence of Different Fixing Methods on Cardiac Index in C57 Mice of Wild Type andβ-adrenoceptor Knockout Mice
10.11842/wst.2014.02.007
- VernacularTitle:不同固定方法对野生型及β受体基因敲除C57小鼠心电指标的影响
- Author:
Xia LI
;
Junhong GAO
;
Jingjun ZHAO
;
Hui BEN
;
Xiaochun YU
;
Bing ZHU
;
Peijing RONG
- Publication Type:Journal Article
- Keywords:
Fixing method;
ST-segment amplitude in ECG;
heart rate;
physiological / pathological states
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):249-253
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to choose the best fixing method of electrocardiogram (ECG) measurement in mice by comparison of ECG index in three different fixing ways. Changes of ST amplitude and heart rate in ECG were measured in the self-made mouse sets, tape bundling or breathing anesthesia state. The amplitudes of the ST seg-ment in C57BL6 mice and β1/β2-AR double knockout mice were elevated in tape bundling or breathing anesthesia state in comparison with that in the self-made mouse sets (all P < 0.01). The heart rate under the tape bundling state in C57BL6 mice was significantly faster than that in the self-made mouse sets (P< 0.05 or P< 0.01). And the heart rate under anesthesia in β1/β2-AR double knockout mice was significantly slower than that in tape bundling state or the self-made mouse sets (both P < 0.01). It was concluded that relative to the binding and anesthesia method, fixing mouse in the self-made mouse sets, which can more truly reflect the physiological or pathological states, is a relatively good fixing method of ECG measurement in experimental study.
