Molecular Cloning and Characterization of HCT (Hydroxycinnamoyl-CoA:Shikiate/Quinate Hydroxycinnamoyltransferase) Gene in Lonicera japonica Thunb.

VernacularTitle:金银花莽草酸/奎宁酸香豆酰转移酶（LjHCT）基因克隆与序列分析
Author: Liu HE ; Xiaolan XU ; Zhenzhong WANG ; Yuan BI ; Wei XIAO ; Hongmei LUO ; Chao SUN
Publication Type:Journal Article
Keywords: 莽草酸/奎宁酸香豆酰转移酶; 金银花; 绿原酸; 基因克隆与序列
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):263-268
CountryChina
Language:Chinese
Abstract: Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase(HCT) is an key enzyme involved in the biosynthesis of chlorogenic acid in Lonicera japonica. In this study, eight putative HCT genes were cloned with RACE (rapid amplification of cDNA ends) technology based on the analysis of transcriptome in L. japonica. Among them, one was suggested as HCT gene (LjHCT) in L. japonica through analysis of sequence similarity, physical and chemical properties, and domain conservation of the proptein. LjHCT gene containing 1 275 bp encodes a protein with the molecular weight of 47 kDa. These results will provide foundation for exploring the function of LjHCT in Lonicera japonica.