Molecular Identification of the Traditional Chinese Medicine of the Deers Using COI Barcode Sequence

VernacularTitle:基于COI条形码的鹿类中药材DNA条形码分子鉴定
Author: Dong LIU ; Qini QIAN ; Hongyin ZHANG ; Dejun ZENG ; Jing JIA ; Hui ZHANG
Publication Type:Journal Article
Keywords: Traditional Chinese medicine of deers; COI barcode sequence; DNA barcoding; identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):274-278
CountryChina
Language:Chinese
Abstract: Objective: Using the COI Barcode to establish the standard method to identify the traditional Chinese medicine of deer products. Methods: In this study, DNA extraction, PCR amplification, and sequencing of experimen-tal samples (deer antler, penis and testis, tendon, tail, bone, foetus) were successful. COI sequence database were constructed and commercial crude drugs of deer were investigated and analyzed. Results: By using universal COI primer,PCR amplification is preferably. All the species could be identified based on COI sequences database of tra-ditional Chinese medicine of deer which contained 101 samples of 8 species. We have collected 40 commercial crude drugs in which 18 samples were identified as species described in Chinese pharmacopoeia. Conclusion: DNA barcode technology based on COI sequence could be a standard approach to identify traditional Chinese medicine of deer products, and provide the basis for the identification of commercial deer medicine.