HPLC Characteristics of Ophiocordyceps sinensis
10.11842/wst.2014.02.012
- VernacularTitle:冬虫夏草高效液相特征图谱分析
- Author:
Zhengming QIAN
;
Peipei SUN
;
Wenqing LI
;
Wenjia LI
;
Jun XU
;
Jing HE
;
Li XIANG
- Publication Type:Journal Article
- Keywords:
Ophiocordyceps sinensis;
Hirsutella sinensis;
Cordyceps militaris;
HPLC;
QTOF
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):279-283
- CountryChina
- Language:Chinese
-
Abstract:
A HPLC-QTOF MS method was established for analysis of components in Ophiocordyceps sinensis . The HPLC analysis was performed on an Agilent Zorbax SB Aq (150 mmí4.6 mm, 5 μm) with gr adient elution (5 mmol·L-1 ammonium acetate aqueous solution-acetonitrile), flow rate was 0.8 mL·min-1 and detection wave-length was 260 nm. The developed method was successfully applied in analysis of three different samples in-cluding O. sinensis, Hirsutella sinensis ( anamorph of O.sinensis) and Cordyceps militaris. Nine compounds were i-dentified in both O.sinensis and H.sinensis, which eight compounds were identified in C.militaris.
