Molecular Identification of Serpentis Periostracum and Its Adulterants Based on COI Sequence
10.11842/wst.2014.02.013
- VernacularTitle:基于COI条形码的中药材蛇蜕及其易混伪品的DNA分子鉴定
- Author:
Linchun SHI
;
Jun CHEN
;
Dong LIU
;
Hongyin ZHANG
;
Jing JIA
;
Hui ZHANG
;
Hui YAO
- Publication Type:Journal Article
- Keywords:
Serpentis Periostracum;
COI;
DNA barcoding;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):284-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study aimed to distinguish Serpentis Periostracum from its adulterants, which will provide the basis for its safe application. Methods: Here, COI sequences of 68 samples from 13 species were PCR amplified and sequenced. Furthermore, the DNA Barcoding Gap and phylogenetic cluster analysis were carried out. Results:The results exhibited that the COI sequences of all the three origin animals of Serpentis Periostracum have DNA Barcoding Gap. For phylogenetic cluster analysis, all the three origin animals showed monophyletic and every species can be discriminated clearly. Conclusion: COI is an effective DNA barcode for the identification of Serpen-tis Periostracum.
