Identification of Inulae Herba, Inulae Flos and Their Closely Related Species Based on ITS2 Sequence
10.11842/wst.2014.02.017
- VernacularTitle:基于ITS2序列鉴定中药材金沸草、旋覆花及其近缘混伪品
- Author:
Licheng GUO
;
Zhigang HU
;
Yuan TU
;
Weidong ZHANG
;
Huizi JIN
;
Wei SUN
;
Haibo HE
;
Xiaoxi MA
;
Bisheng HUANG
- Publication Type:Journal Article
- Keywords:
Inulae Herba;
Inulae Flos;
ITS2 sequence;
identification;
closely related species
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):307-312
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to identify Inulae Herba, Inulae Flos and their closely related species using the ITS2 bar-code, and secure the quality and clinical curative effect of these medicinal materials. DNA was extracted from Inula linariifolia, I. japonica, I. britanica, which are the original species of Inulae Herba and Inulae Flos, together with their closely related species. The ITS2 sequence was amplified by PCR and sequenced bidirectionally. Sequence assembly and generation of consensus sequence were conducted by the CodonCode Aligner. The genetic distances were comput-ed using MEGA 5.0 in accordance with the Kimura-2-parameter (K2P) model, and the phylogenetic tree constructed by the neighbor-joining (NJ) method. The results showed that the ITS2 sequences of the various species have stable variable sites. The intraspecific genetic distances among Inulae Herba and Inulae Flos were obviously lower than the interspecific genetic distance among the above two medicinal materials and its adulterants. The NJ tree based on ITS2 sequences can clearly differ from Inulae Herba, Inulae Flos and their closely related species. It is concluded that ITS2 sequence can be used as DNA barcode to identify Inulae Herba, Inulae Flos and their closely related species.
