Highly Specific DNA Barcoding Primers of ITS2 for Gastrodia elata

VernacularTitle:药材天麻ITS2特异性引物的筛选
Author: Xiaoxi MA ; Wei SUN ; Qini QIAN ; Weichao REN ; Lan WU ; Yaqin ZHANG ; Ming SONG
Publication Type:Journal Article
Keywords: Gastrodia elata; ITS2; specific primers; identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):325-328
CountryChina
Language:Chinese
Abstract: This study aimed to design a pair of primers for amplifying internal transcribed spacer 2 region which was used to identify Gastrodia elata through optimizing of DNA extraction and PCR amplification process. Two sequences are used for research object by amplification of universal primers. Design three pairs of specific primers by Primer Premier 5.0 and select the highest specificity through the study of 22 samples. The results showed that identification efficiency of the primer named TM2F-2R is as high as 90.9% when Annealing Temperature is equal to 54 degrees Celsius. Therefore, TM2F-2R can be used as primers ITS2 sequences of G. elata, this article provides a set of accu-rate and stable identification methods for G. elata in the molecular identification.