Identification of Anemone raddeana and Its Adulterants by ITS2 Sequence

VernacularTitle:基于ITS2条形码序列鉴定中药材两头尖及其混伪品
Author: Weichao REN ; Wei MA ; Chao AN ; Xiaoxi MA ; Lan WU ; Wei SUN
Publication Type:Journal Article
Keywords: A nemone raddeana; ITS2; identification; adulterants
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):301-306
CountryChina
Language:Chinese
Abstract: In this study, the internal transcribed spacer 2 of nuclear ribosomal DNA (ITS2) sequence was used for i-dentifying A nemone raddeana and its adulterants to ensure the quality of medicines and clinical efficacy. Genomic DNA was extracted from 36 samples using Genomic DNA kit and used as templates for Polymerase Chain Reaction (PCR). Sequence assembly and consensus sequence generation were performed by CodonCode Aligner. The intraspe-cific and interspecific genetic distances were computed and the neighbor-joining tree was constructed by MEGA 5.1 in accordance with the Kimura 2-Parameter (K-2P) model. Results: The length of ITS2 sequence of A nemone rad-deana was 216 bp. The Maximum intraspecific genetic distance was 0.014, the minimum interspecific genetic dis-tance was 0.021. The NJ tree showed that A . raddeana differ from its adulterants obviously. Conclusion: ITS2 se-quence was able to identify A . raddeana and its adulterants correctly stably and correctly, which provides a new tech-nique to its identification.