Effect of interferon on HEL cell apoptosis and JAK2 V617F mutation gene expression
10.3969/j.issn.1006-5725.2014.06.015
- VernacularTitle:干扰素对人红白血病HEL细胞凋亡及JAK2 V617F突变基因表达的影响
- Author:
Liya YUAN
;
Hong LI
;
Jiping RONG
;
Zhu WEN
;
Huo YU
;
Zhigang WANG
;
Guoan CHEN
;
Dexiang JI
;
Xianbao HUANG
;
Wei LU
- Publication Type:Journal Article
- Keywords:
Leukemia;
Interferon;
HEL cells;
Apoptosis;
JAK2 V617F mutation gene
- From:
The Journal of Practical Medicine
2014;(6):882-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Interferon-α2b on HEL cells (human erythroleukemia cell line) growth, apoptosis and JAK2 V617F mutation gene expression. Methods HEL cells were placed in RPMI1640 containing 10% FBS and incubated in a cell incubator. Cells in the logarithmic growth phasem were collected, adjusting the cell density to 1 × 105/mL for experimental research. The interferon concentration in five groups were 0, 5 × 105, 10 × 105, 50 × 105, 100 × 105 U/L, with different incubation time (0, 24, 72, 120 h), respectively. The cell growth status in different groups was observed in the inverted optical microscope; MTT was used to detect the inhibition of interferon on HEL cell proliferation. Cell apoptosis was detected by flow cytometry. Fluorescence quantitative PCR was used to detect the mutation gene of JAK2 V617F expression. Results Inhibition rates of Interferon on the HEL cell proliferation in 5 × 105 U/L, 10 × 105 U/L, 50 × 105 U/L, 100 × 105 U/L groups were 18.57%, 25.10%, 42.10%, 57.00%, respectively. JAK2 V617F/GAPDH by fluorescence quantitative was 1.556, 1.213, 0.870 respectively under the concentration of interferon 100 × 105 U/L for 24, 72, 120 h. Conclusions Interferon-α2b can inhibit HEL cells proliferation and induce HEL cells apoptosis. Increasing concentration of interferon increases HEL cell apoptosis rate. Interferon can inhibit JAK2 V617F expression of HEL cells in a dose-dependent manner.
