Online Two Dimensional Liquid Chromatography and Its Application on TCM Quality Control Analysis
10.11842/wst.2014.03.018
- VernacularTitle:在线二维液相色谱技术及其在中药质量控制中的应用与展望
- Author:
Rong AN
;
Yao XIAO
- Publication Type:Journal Article
- Keywords:
Online 2D-LC;
online comprehensive 2D-LC;
heart-cut 2D;
peak capacity;
TCM analysis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(3):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Separation of complex mixtures needs more resolving power. In two dimensional liquid chromatography (2D LC), the sample is subjected to two different separation conditions or mechanisms, which significantly increases the peak capacity,resolving power and reduces peak overlap. Meanwhile, 2D separation is more reliable for peak i-dentification from run to run than single-dimension chromatography, because two independent elution times are ob-tained for each peak. Among different 2D liquid chromatographic combinations/configurations, online comprehensive 2D-LC has appeared to be very attractive due to its high peak capacity, minimal sample loss, good reproducibility, and easy to automation, especially for separation of very complex samples. The present review discusses the theoreti-cal aspects and hardware configuration of online 2D-LC, particularly online comprehensive 2D-LC. It highlights its concept, features, advantages, applications and prospective in TCM quality Control analysis.
