Study on Volatile Ingredient and GC-MS Fingerprint of Bu-yang Huan-wu Decoction

VernacularTitle:补阳还五汤挥发性成分指纹图谱的研究
Author: Xuyang LONG ; Heng FANG ; Fashan WANG ; Jianmin LI ; Yandong REN ; Ning ZHANG
Publication Type:Journal Article
Keywords: Bu-yang Huan-wu decoction; GC-MS; fingerprint; volatile ingredient
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(3):560-564
CountryChina
Language:Chinese
Abstract: This study was aimed to establish GC-MS fingerprint of Bu-yang Huan-wu (BYHW) decoction. The 5HP-MS quartz capillary column was used. The temperature program carrier gas was He. The velocity was 1.0 mL·min-1. The injection volume was 1 μL. The split ratio was 1:20. The MS conditions were electron impact (EI) ion source with the transmission line temperature at 280oC, ion source temperature at 230oC, and quadrupole temperature at 150oC. The mass scan range was m/z 50~550. And the results were contrasted with ChemStation and NIST 05a. The results showed that nine common peaks were obtained in the fingerprint of eleven batches of BYHW decoction by peaks matched with the Similarity Evaluation System for Chromatographic Fingerprint (2004 A Version). The similari-ty of fingerprints was calculated more than 0.93. It was concluded that fingerprinting can be used as a method of quality control and provide evidence for the research of volatile ingredient of BYHW decoction.