Identification of Gekko geeko Linnaeus and Adulterants Using the COI Barcode

VernacularTitle:基于COI条形码序列的蛤蚧及其混伪品的DNA分子鉴定
Author: Hongyin ZHANG ; Linchun SHI ; Dong LIU ; Jing JIA ; Jun CHEN ; Hui ZHANG
Publication Type:Journal Article
Keywords: Gekko gecko Linnaeus; COI; DNA barcoding; identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):269-273
CountryChina
Language:Chinese
Abstract: DNA barcoding technology is widely opplied for species identification and discovery by using short and standard fragments of DNA sequences. In this study, the cytochrome c oxidase subunit 1 (COI) sequence as a DNA barcode was used to identify the Gekkogecko Linnaeus medicinal materials and adulterants in order to provide a new identification method of G. gecko Linnaeus. Genomic DNA was extracted from the experimental samples. The COI regions of nrDNA were amplified using polymerase chain reaction and sequenced bidirectionally. The alignment and NJ tree construction were performed in MEGA6.0. COI sequences can be obtained from all samples. The average intra-specific K2P distance of G. gecko Linnaeus was 0.005 and the maximum intra-specific distance was 0.013. All the G. gecko Linnaeus medicinal samples clustered into a clade in the NJ tree and can be distinguished from the adulterants. In a conclusion, COI can be used to correctly identify G. gecko Linnaeus, and it will be a potential DNA barcode for identification of other animal medicinal materials.