HPLC Simultaneous Determination of Sodium Danshensu, Hesperidin and Salvianolic ;Acid B in Niaosaitong Capsules
10.3969/j.issn.1005-5304.2014.06.026
- VernacularTitle:HPLC同时测定尿塞通胶囊中丹参素钠、橙皮苷及丹酚酸B含量
- Author:
Shaoqing WANG
;
Xing JIN
;
Qingbo ZHANG
- Publication Type:Journal Article
- Keywords:
Niaosaitong Capsules;
sodium Danshensu;
hesperidin;
salvianolic acid B;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(6):83-85
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To establish an HPLC method for determination of sodium Danshensu, hesperidin and salvianolic acid B in Niaosaitong capsules. Methods Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength was set at 286 nm. Results Sodium Danshensu, hesperidin and salvianolic acid B had good linearity in the range of 0.038 44-1.922 μg, 0.000 207 052 8-4.044 μg, 0.034 28-1.714 μg respectively, and the average recovery rate was 90.9%-107.1%, RSD was 2.1%-3.2%. Conclusion The methods is simple and accurate, and can be used for the quality control of Niaosaitong capsules.
