Using ITS2 Sequence to Identify Descurainiae, Lepidii Semen and Its Adulterants
10.11842/wst.2014.02.014
- VernacularTitle:基于ITS2序列鉴定南北葶苈子药材及其混伪品
- Author:
Yuan TU
;
Bo ZHAO
;
Fang WEN
;
Wei SUN
;
Ming SONG
;
Haibo HE
;
Zhigang HU
;
Licheng GUO
;
Xiuqiao ZHANG
- Publication Type:Journal Article
- Keywords:
Descurainiae and Lepidii Semen;
ITS2 sequence;
identification;
adulterants
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):288-294
- CountryChina
- Language:Chinese
-
Abstract:
Descurainiae, Lepidii Semen and their it adulterants were identified by analysising their ITS2 sequences. The genomic DNA was extracted from 46 samples including Descurainiae and Lepidii Semen and their it adulterants. Their ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using CodonCode Aligner v 4.25. The genetic distances, variable sites and the neighbor-joning (NJ) phylogenetic tree were computed by MEGA 6.0 in accordance with the Kimura 2-parameter (K2P) model. The results showed that the intra-specific genetic distances of Descurainia sophia and Lepidium apetalum were 0.021 and 0.010, which were smaller than inter-specific ones of D. sophia, L. apetalum and their adulterants. The NJ tree showed that both D. sophia and L. apetalum were clustered into one monophyletic branch, and clearly separated with their sibling species. Therefore ITS2 sequence was able to identify Descurainiae and Lep-idii Semen and its adulterants to ensure the quality of medicines and clinical efficacy.
