Identification of Xanthii Fructus and Its Adulterants Based on ITS2 Sequence
10.11842/wst.2014.02.021
- VernacularTitle:苍耳子药材及其混伪品ITS2序列鉴定研究
- Author:
Jun WANG
;
Xia LIU
;
Yaqin ZHANG
;
Ming SONG
;
Yunhan LIN
;
Xiaoxi MA
;
Wei SUN
;
Li XIANG
;
Zhigang HU
;
Lan WU
;
Xueqiong ZHANG
;
Weiyi HU
- Publication Type:Journal Article
- Keywords:
Xanthii Fructus;
DNA barcoding;
ITS2;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(2):329-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify Xanthii Fructus and secure its quality and safety in medication. Methods: Total ge-nomic DNA was extracted from Xanthii Fructus and its adulterants. ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V 4.2. The Kimura 2-Parameter (K2P) distances were calculated using MEGA 5.0. The neigh-bor-joining (NJ) phylogenetic trees were constructed. Results: The intraspecific genetic distances of Xanthii Fructus were 0. The interspecific genetic distances between Xanthii Fructus and its adulterants were ranged from 0.009 to 0.542. The NJ tree showed that Xanthii Fructus could differ from its adulterants obviously. Conclusion: ITS2 can be used to identify Xanthii Fructus from its adulterants effectively, and our study further confirmed the effectiveness of ITS2 to identify traditional Chinese medicinal materials.