Effect of Wuzi Yanzong Fang Against Cyclophosphamide Induced DNA Damage in Mice
10.3969/j.issn.1005-5304.2014.06.014
- VernacularTitle:五子衍宗方对环磷酰胺致小鼠DNA损伤的影响
- Author:
Miaomiao LIU
;
Ding YUAN
;
Weifeng HUANG
;
Ben PENG
;
Changcheng ZHANG
- Publication Type:Journal Article
- Keywords:
Wuzi Yanzong Fang;
cyclophosphamide;
DNA damage;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(6):38-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study protective effects of Wuzi Yanzong Fang on DNA damage induced by cyclophosphamide (CTX) in mice, and explore its mechanism. Methods BalB/c mice were randomly divided into normal group, model group, Wuzi Yanzong Fang low dose group and Wuzi Yanzong Fang high dose group. Mice in Wuzi Yanzong Fang groups were pretreated with Wuzi Yanzong Fang for 7 days, then the mice in Wuzi Yanzong Fang groups and model group were intraperitoneally injected with CTX (100 mg/kg) every other day for three times, and mice in Wuzi Yanzong Fang groups were continued administered with Wuzi Yanzong Fang. Animals were sacrificed in twelve hours after the final treatment of CTX. ELISA was used to detect 8-OHdG content in serum, and single cell gel electrophoresis to detect DNA damage in bone marrow cells. Results Wuzi Yanzong Fang low dose group and high dose group reduced the level of 8-OHdG in serum. Wuzi Yanzong Fang significantly decreased Olive tail moment, tail moment, tail length and tail DNA%in mouse bone marrow cells. Conclusion Wuzi Yanzong Fang has good protective effects on DNA damage caused by CTX.
