Effects and mechanism of hypoxia on the growth and invasion of human cervical cancer Hela cells
10.3969/j.issn.1006-5725.2014.04.007
- VernacularTitle:低氧对宫颈癌Hela细胞生长侵袭能力的影响及机制研究
- Author:
Peng KOU
;
Qin XU
;
Jianjun LIU
;
Jing YANG
;
Lihua YANG
- Publication Type:Journal Article
- Keywords:
Cervical neoplasms;
Hela cells;
Hypoxia;
Hypoxia inducible factor-l;
Vimentin
- From:
The Journal of Practical Medicine
2014;(4):522-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of hypoxia on the growth and invasion of human cervical cancer Hela cells. Methods Hypoxia microenvironment was mimicked by cobalt chloride (CoCl2), in which the growth of Hela cells were determined by MTT method, the migration ability was detected by scratch test, and the expressions of HIF-1αand Vimentin protein were measured by immunofluorescence. Results The growth of Hela cells treated with 150 μmol/L of CoCl2 for 6 h was promoted. The migration ability in hypoxia group at 48 h was significantly higher than that in ordinary oxygen group. The expressions of HIF-lαand Vimentin in Hela cells in hypoxia group were significantly higher than those in ordinary oxygen group. There was significantly positive correlation between the expressions of HIF-1α and Vimentin (P < 0.05). Conclusion Hypoxia could promote the expression of HIF-lα, which then could up-regulate the expression of Vimentin , and finally enhanced the ability of invasion and metastasis of Hela cells.
