Pro-Apoptosis Effects of Danhong Injection on the SMMC-7721 Hepatoma Cells
10.3870/yydb.2014.05.004
- VernacularTitle:丹红注射液对SMMC-7721肝癌细胞的促凋亡作用
- Author:
Lei ZHONG
;
Liping XU
;
Xianzhong QIAN
;
Huijing JIN
;
Chao CHEN
;
Lijun WU
- Publication Type:Journal Article
- Keywords:
Danhong injection;
SMMC-7721 hepatoma cells;
Apoptosis
- From:
Herald of Medicine
2014;(5):565-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of danhong injection on the apoptosis of SMMC-7721 heptoma cells.Methods The hepatocellular carcinoma cell line SMMC-7721 was incubated with different concentrations(0,2,4,8 μL·mL-1) of danhong injection, and cell morphology was studied under the microscope. The apoptosis index was detected by flow cytometry at 0,6,12,24 h after cultured with 2 μL·mL-1danhong injection. Cell proliferation was measured by MTT assay;Gene expressions of p53,Bax and Bcl-2 were detected by RT-PCR at different drug concentrations. Results The morphology of the hepatoma cells changed obviously,and the apoptosis index increased by danhong injection in a dose-dependant manner. The danhong injection decreased gene expressions of Bcl-2,and obviously increased p53 and Bax. Conclusion Danhong injection can dose-and time-dependently promote apoptosis of SMMC-7721 cells.
