Determination of 5 New Abused Drugs in Plasma by In-tube Solid-phase Microextraction Coupled to Liquid Chromatography Tandem Mass Spectrometry
10.3870/yydb.2014.05.007
- VernacularTitle:毛细管固相微萃取-液质联用测定血浆中5种新型滥用药物
- Author:
Yi WANG
;
Ziqiu LIU
- Publication Type:Journal Article
- Keywords:
In-tube solid-phase microextraction;
Liquid chromatography tandem mass spectrometry;
Abused drugs,new type;
Plasma
- From:
Herald of Medicine
2014;(5):575-578
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of new abused drugs in plasma by using ultra-high performance liquid chromatography tandem mass spectrometry ( UPLC-MS/MS ) combined with in-tube solid-phase microextraction. Methods 2 mL of borax buffer was added to 1 mL of plasma with drugs. Then,the mixture was withdrawn into a syringe connected to a stainless steel needle welded with DB-1701 capillary column. 50 μL acetonitrile was used as elution solvent at the flow rate of 300 μL·min-1 for 2 min. Subsequently,the drugs on elute were determined by using UPLC-MS/MS in positive mode with multiple reaction monitoring( MRM) to determine drug concentration in human plasma samples. ResultsThe recoveries rates of the five abused drugs ( codeine hydrochloride, tramadol, diphenoxylate hydrochloride, diazepam, and triazolam) in plasma were between 69. 2% -81. 7% with the RSDs less than 10%. LODs of the drugs were less than 25 ng·mL-1 . Seven out of the 26 plasma samples were determined positive. Conclusion The in-tube SPME method coupled to UPLC-MS/MS is simple, rapid, and environmental friendly, which is suitable for the the rapid screening of the new abused drugs.
