Drug Resistance Mechanism of Patients Infected with Aminoglycoside-resistant Acinetobacter Baumannii in Emergency Intensive Care Unit
10.3870/yydb.2014.05.008
- VernacularTitle:急诊重症监护病房感染患者耐氨基苷鲍曼不动杆菌的耐药机制
- Author:
Xue ZHAO
;
Peitao YU
;
Zhijun XU
;
Qing GU
;
Lifeng QIU
;
Yi WANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Aminoglycoside-passivating enzymes genes;
16S rRNA methylase gene;
Intensive care unit,emergency
- From:
Herald of Medicine
2014;(5):579-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate drug resistance mechanism of aminoglycoside-resistant Acinetobacter baumannii by detecting 16S rRNA methylase gene and three common genes of aminoglycoside-modifying enzymes in Acinetobacter baumannii infected patients at EICU. Methods The 48 Acinetobacter baumannii strains were collected,and antimicrobial susceptibility tests were performed by VITEK automicroscan. The MIC was detected by 2-fold agar dilution method,and genes were analyzed by polymerase chain reaction( PCR) . Results Among 48 strains,28 were highly resistant to aminoglycosides and 20 showed lower resistances. The 16S rRNA armA,APH(3')-I,ANT(3'')-Ia,AAC(6')-Ib genes were detected in 71. 43%,60. 71%,82. 14%, and 53. 57%of the 28 highly resistant strains,but only present in 0. 00%,0. 05%,0. 05%,and 0. 05%of the low-resistant isolates(P<0. 01). Conclusion The aminoglycoside-modifying enzymes and 16S rRNA methylase were frequently found in Acinetobacter baumannii clinical isolates,which is closely related to the high-level resistance to aminoglycoside antibiotics.
