A study on characteristics of rifampin-and isoniazid-resistance genes of Mycobacterium tuberculosis in Ganzhou region
10.3969/j.issn.1673-4130.2014.09.020
- VernacularTitle:赣州地区结核分枝杆菌利福平和异烟肼耐药基因突变特点的研究
- Author:
Yijun LUO
;
Jianlin YANG
- Publication Type:Journal Article
- Keywords:
mycobacterium tuberculosis;
extensively drug-resistant tuberculosis;
mutation
- From:
International Journal of Laboratory Medicine
2014;(9):1136-1137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of rifampin(RFP) and isoniazid(INH) resistance genes of Mycobacteri-um tuberculosis in Ganzhou region .Methods Gene chip technology was employed to detect common resistance genes in 130 sputum samples with smear positive acid-fast staining and Ct values less than 30 .Results Among 130 samples ,34 samples(26 .2% ) were found resistance ,including 30 samples(23 .1% ) resistant to RFP ,26 samples(20 .0% ) resistant to INH ,and 21 samples(16 .2% ) resistant to both .The mainly mutation of RFP resistant gene was rpoB gene 531(C→ T ) ,accounting for 42 .4% ,while the mainly mutation of INH resistant gene was katG gene 315(AGC→ACC) ,accounting for 65 .4% .Conclusion The mutation charateristics of RFP and INH resistance-associated genes of Mycobacterium tuberculosis in Ganzhou region is consistent with domestic and foreign research .
