Isolation,culture, adipocgenic and osteogenic induction of Tupaia bone marrow mesenchymal stem cells
10.3969/j.issn.1671.7856.2014.003.003
- VernacularTitle:树鼩骨髓间充质干细胞体外分离培养及成脂成骨诱导
- Author:
Caixia LU
;
Xiaofei LI
;
Wenguang WANG
;
Xiaomei SUN
;
Pinfen TONG
;
Dexuan KUANG
;
Jiejie DAI
- Publication Type:Journal Article
- Keywords:
Tupaia;
Bone marrow mesenchymal stem cells;
Cell culture;
Adipogenic induction;
Osteogenic induction
- From:
Chinese Journal of Comparative Medicine
2014;(3):10-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the isolation,culture, adipogenic and osteogenic induction Tupaia bone marrow mesenchymal stem cells(BM-MSCs).Method The BM-MSCs from tupaia were isolated and expended by combination of gradient centrifugation and adherence culture , then subcultured and observed for morphology under inverted phase contrast microscope.BM-MSCs were induced to adipocytes .and osteoblasts in vitro Result Cells were spindle or triangle-shaped, and clone proliferation .Cells were successfully induced into adipocytes .and osteoblasts Conclusions The method of isolation BM-MSCs from tupaia by combination of gradient centrifugation and adherence culture is simple and feasible , BM-MSCs have differentiation potential into adipocytes and osteoblasts .
