Establishment of two cardiac-specific human cardiac troponin C mutation transgenic mice and comparative analysis
10.3969/j.issn.1671.7856.2014.003.014
- VernacularTitle:人心肌肌钙蛋白C两种突变转基因小鼠模型建立与对比分析
- Author:
Shan GAO
;
Wei CHEN
;
Ning LIU
;
Wenping GE
;
Xiang GAO
;
Dan LU
;
Lianfeng ZHANG
;
Wei DONG
- Publication Type:Journal Article
- Keywords:
Cardiac troponin C;
Hypertrophic cardiomyopathy;
Dilated cardiomyopathy;
Transgenic mice;
Echocardiography
- From:
Chinese Journal of Comparative Medicine
2014;(3):67-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To established cardiac-specific transgenic mice of the cTnC D145E and cTnCG159D and compare the HCM and the DCM.Methods The cTnCD145E and cTnCG159D were generated by site-directed mutagenesis and the transgenic plasmids were constructed by insertion of the mutant genes under the control of α-MHC, which is a myocardium specific promoter.The transgenic mice were generated by microinjection and were all maintained on a C57BL/6J genetic backgroud .The cardiac structure and function of the transgenic mice were compared and analysized by echocardiographic and pathological observation at different ages .Results The cTnCD145E and cTnCG159D transgenic mice were established and developed to HCM and DCM, respectively, with aging.The left ventricular end-systolic volume (ESV) and left ventricular end-diastolic volume ( EDV) decreased and ejection fraction ( EF) and left ventricular end-systolic posterior wall thickness (ESPWT) increased in the cTnCD145E transgenic mice, while EDV and ESV increased and EF and ESPWT decreased in the cTnCG159D transgenic mice at 12 months of age.Conclusions Cardiac-specific human cTnCD145E transgenic mice showed HCM phenotypes , and cardiac-specific human cTnC G159D transgenic mice showed DCM phenotypes , which can be used as different models for comparative study of the pathogenesis of cardiomyopathy .
