Expression characteristic of IFNGR in stellate ganglion of female goats

VernacularTitle:γ-干扰素受体在雌性山羊星状神经节的表达
Author: Qiang LI ; Zhihao WANG ; Xiufang JIN ; Yongping XU ; Xiao GUO ; Wendong CHEN ; Wei DONG
Publication Type:Journal Article
Keywords: Interferon-γreceptors; Stellate ganglion; Immunohistochemical SP method; PCR; Goats
From: Chinese Journal of Immunology 2014;(5):604-608
CountryChina
Language:Chinese
Abstract: Objective:To detect the existence of IFNGR in the stellate ganglion in female goats .Methods:The stellate ganglia were taken from female goats .The expression of IFNGR was detected by PCR method and immunohistochemical SP staining .Results:The results showed that:IFNGR immunoreactive substances were distributed in neurons , supporting cells and passing fiber , and mainly in the cytomembrane and nuclei of neurons .The relative expression of IFNGR was very significantly higher than that of non-neuronal cells (P<0.01).The sequence of IFNGR1 gene amplified by PCR method contained 376 bp, and IFNGR1 gene of goats exhibited the highest homology with sheep(98%),followed by cattle(97%).Conclusion:The results suggested that the IFNGR in the Stellate Gan-glion of female goats mainly expressed and located in sympathetic postganglionic neurons which were provided with the conditions for the role of IFN-γ, which implied that the Stellate Ganglion may act as the critical point to concert the immune regulation of IFN -γand neu-roregulation of autonomic nerve on cardiovascular system .