IL-33 transgenic mice increase the myeloid differentiation in hematopoietic stem cells
10.3969/j.issn.1671.7856.2014.003.009
- VernacularTitle:IL-33转基因小鼠骨髓造血干／祖细胞向髓系细胞分化的能力增强
- Author:
Lin BAI
;
Guiying SHI
;
Shan GAO
;
Yajun YANG
;
Kun GAO
;
Lianfeng ZHANG
- Publication Type:Journal Article
- Keywords:
IL-33;
Flow cytometry;
Hematopoietic stem cell;
Myeloid
- From:
Chinese Journal of Comparative Medicine
2014;(3):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of IL-33 on the Hematopoietic stem cells and progenitor cells . Methods Cells from the peripheral blood , spleen, thymus and bone marrow were stained with indicated antibodies and analyzed by flow cytometry . The LT-HSCs were sorted and culture using in vitro clonogenic assay . Results The percentage of B cells and T cells was decreased and the percentage of M cells was increased in the peripheral blood from IL -33 transgenic mice .Compared with the wildtype mice , the number of HSCs , MPPs and CLP was decreased;meanwhile the number of CMP and GMP was increased in the bone marrow from IL-33 transgenic mice .An in vitro clonogenic assay showed that LT-HSCs increased the ability to self-renew from IL-33 transgenic mice .And the percentage of S-G2-M stage hematopoietic stem cell was increased from IL-33 transgenic mice .Conclusion IL-33 increase the myeloid differentiation in hematopoietic stem cells .
