Developmental bisphenol-A exposure affects hippocampal dentate gyrus area spine formation through Wnt/β-catenin signaling

VernacularTitle:发育期双酚A暴露通过Wnt信号通路影响海马齿状回区突触形成
Author: Zhihua LIU ; Huili WANG ; Sheng WU ; Yang LIU ; Xiangtao CHEN
Publication Type:Journal Article
Keywords: bisphenol A; dendritic spine; Wnt signaling; synapse formation
From: Chinese Journal of Pharmacology and Toxicology 2014;(2):161-167
CountryChina
Language:Chinese
Abstract: OBJECTIVE To investigate the effects and its underlying mechanis m of bisphenol-A (BPA)exposure on spine and synapse formation in detate gyrus (DG)area of hippoca mpus during criti-cal develop mental period.METHODS Sprague-Dawley(SD)rats were injected intraperitoneally with BPA (50,250 and 500 μg·kg -1·d -1 )fro m postnatal day 7 (PND7)to PND14.Dendritic spine morphol-ogy in DG area was exa mined using Golgi-Cox staining method and determined with I mage J software. Western blotting method was e mployed to test the Wnt related proteins.RESULTS The spine density and the average spine head size in BPA exposed groups significantly decreased in a dose-dependent manner when co mpared to control group(P<0.05).Meanwhile,Wnt related proteins were affected dur-ing BPA exposure.Specifically,the percentage of phosphorylated β-catenin increased following BPA ex-posure (P<0.05),whereas Wnt7a expression level was significantly decreased and Wnt5a expression level increased (P<0.05).CONCLUSION Wnt signaling pathway plays an i mportant role in BPA-in-duced i mpairments in spine and synapse formation.