Study the roles of KLF2, KLF4 in prediction of acute lung injury in rats induced by endotoxin
10.3760/cma.j.issn.1671-0282.2014.05.013
- VernacularTitle:KLF2、KLF4预测内毒素所致急性肺损伤大鼠的研究
- Author:
Yong YANG
;
Youkui SHEN
;
Liwen DONG
;
Zhengqing LOU
;
Jun WANG
;
Xiaoqing FU
- Publication Type:Journal Article
- Keywords:
KLF2;
KLF4;
(Ae) lung injury;
Endotoxin;
Early diagnosis
- From:
Chinese Journal of Emergency Medicine
2014;23(5):521-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of KLF2 mRNA and KLF4 mRNA in the acute lung injury (ALl) rats induced by lipopolysaccharide (LPS),and to analyze the correlation between KLF2,KLF4 and ALI.Methods A total of 100 SD rats were randomly divided into 2 groups:normal control group and LPS treated group,then the latter group was randomly further divided into 3 subgroups as per the serum and lung tissue samples taken separately at 2,4 and 24h after modeling.The ALI model was made by injecting 5mg/kg LPS into tail vein.The pathological changes of lung tissue were observed in each group,and the expressions of KLF2,KLF4 mRNA in serum and lung tissue were detected by RT-PCR.The data of laboratory findings were analyzed with SPSS 17.0 software for statistical analysis.Results The histopathological changes showed the most obvious damage of lung tissue occurred at 4 hours after modeling.The expressions of KLF2 mRNA and KLF4 mRNA in the lung tissue and serum of control group were significantly higher compared to LPS treated subgroups (P ＜0.01).The expression of KLF2 mRNA in LPS treated subgroup at 2 hours was lower than that in LPS subgroups at 4 hours and 24 hours (P ＜ 0.01),while the expression of KLF4 mRNA in LPS treated subgroup at 4 hours was lower than that in LPS treated subgroups at 2 hours and 24 hours (P ＜ 0.01).Conclusions The expression of KLF2 mRNA was occurred earlier than the pathological changes in acute lung injury,while the expression of KLF4 was emerged synchronously,and both KLF2 and KLF4 could be used as candidates of predictive and diagnostics molecular markers of ALI.
