Effects of hydrogen sulfide on high glucose-induced injury of mouse glo-merular podocytes
10.3969/j.issn.1000-4718.2014.04.017
- VernacularTitle:硫化氢对高糖诱导的小鼠足突细胞损伤的影响
- Author:
Ye LIU
;
Li PENG
;
Shengxia LU
;
Yuejuan DU
;
Yuantao LIU
;
Yuqin FU
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Hydrogen sulfide;
Podocytes;
Cystathionine γ-lyase;
Zonula occludens-2 protein;
β-catenin
- From:
Chinese Journal of Pathophysiology
2014;33(4):670-674
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of hydrogen sulfide ( H2 S) on high glucose ( HG)-induced injury of the mouse podocyte cell line MPC5.METHODS: The cultured MPC5 cells were randomly divided into 4 groups: HG group, normal glucose (NG) group, NG+DL-propargylglycine (PPG) group, and HG+NaHS group.After treated for a certain time, the cells were collected for further detection .The expression of zonula occludens-2 (ZO-2), nephrin,β-cate-nin and cystathionine γ-lyase ( CSE) was determined by Western blotting .RESULTS:High glucose significantly reduced the expression of nephrin, ZO-2 and CSE (P<0.05), while the level of β-catenin was elevated obviously (P<0.05), all in a time-dependent manner.NG+PPG inhibited the levels of ZO-2 and nephrin significantly (P<0.05), and increased the level of β-catenin (P<0.05), all in a PPG concentration-dependent manner.HG+NaHS induced a more significant increase in the levels of ZO-2 and nephrin as compared with HG group (P<0.01), whereas a severe reduction of β-cate-nin in HG+NaHS group was observed as compared with HG group .Compared with NG group , the expression of ZO-2 and nephrin was decreased obviously , and the level of β-catenin was increased in HG +NaHS group.CONCLUSION:Down-regulation of CSE contributes to hyperglycemia-induced podocyte injury .Exogenous H 2 S protects against hyperglycemia-in-duced podocyte injury , possibly through up-regulation of ZO-2 and subsequent suppression of Wnt/β-catenin pathway .
