miR-193 regulates proliferation of rat marrow mesenchymal stem cells by cell cycle-related proteins

VernacularTitle:miR-193通过细胞周期相关蛋白调控大鼠骨髓间充质干细胞的增殖能力
Author: Chenxi MAO ; Di LU ; Chengchao SUN
Publication Type:Journal Article
Keywords: miR-193; Bone marrow mesenchymal stem cells; Cyclin-dependent kinase 2
From: Chinese Journal of Pathophysiology 2014;33(4):645-650
CountryChina
Language:Chinese
Abstract: AIM:To investigate the effects of microRNA-193 (miR-193) on the proliferation and apoptosis of rat bone marrow mesenchymal stem cells (MSCs).METHODS:Cultured rat MSCs were transfected with pre-miR-193 or anti-miR-193 to regulate the expression of miR-193.The proliferation of the MSCs after transfection was evaluated by MTS assay, colorimetric BrdU cell proliferation assay and Ki-67 immunostaining.Cell apoptosis was analyzed by flow cytometry with Annexin V/PI staining.The effect of miR-193 on the expression of cell cycle-related proteins was evaluated by qRT-PCR.RESULTS:Transfection of pre-miR-193 or anti-miR-193 regulated the expression of miR-193 in MSCs effectively . Over-expression of miR-193 significantly promoted the proliferation of MSCs ( P<0.05 ) , and inhibition of miR-193 re-duced the proliferation of MSCs (P<0.05).miR-193 had no significant effect on the apoptosis of MSCs (P>0.05).The result of qRT-PCR indicated miR-193 promoted the expression of cyclin-dependent kinase 2 ( CDK2 ) significantly ( P<0.01).CONCLUSION:miR-193 promotes the proliferation of MSCs possibly through the CDK 2 pathway.