Expression and function of calcium-sensing receptor in mouse embryonic stem cells
10.3969/j.issn.1000-4718.2014.04.014
- VernacularTitle:小鼠胚胎干细胞钙敏感受体的表达和其对细胞增殖的影响
- Author:
Jian SUN
;
Jinfeng YU
;
Hairong LUAN
;
Shuang LI
;
Xiaoyi XU
;
Zhipeng HE
;
Tao WEI
;
Li LI
;
Changqing XU
- Publication Type:Journal Article
- Keywords:
Calcium-sensing receptor;
Mouse embryonic stem cells;
Cell proliferation
- From:
Chinese Journal of Pathophysiology
2014;33(4):651-657
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the functional expression of calcium-sensing receptor (CaSR) in the mouse em-bryonic stem cells (mESCs).METHODS:The expression and distribution of CaSR were detected by Western blotting and immunofluorescence observation in 129 mouse ES-D3 cells.The intracellular concentration of free calcium ([Ca2+]i) was determined by confocal laser scanning microscopy .The cell viability was analyzed by MTT assay and flow cytometry .RE-SULTS:CaSR protein was expressed in mESCs .Extracellular calcium or neomycin significantly increased the expression of CaSR and [Ca2+]i.Neomycin increased the cell viability , up-regulated the protein expression of p-ERK2.These effects of neomycin were inhibited by NPS2390.CONCLUSION:CaSR is expressed in mESCs .The activation of CaSR is involved in the proliferation of mESCs .
