Role of 17β-estradiol in regulation of MIF expression in endometrial stro-mal cells of endometriosis
10.3969/j.issn.1000-4718.2014.04.025
- VernacularTitle:雌激素对子宫内膜异位症在位内膜间质细胞巨噬细胞移动抑制因子表达的调控作用
- Author:
Mingzhen WANG
;
Yuehong QIU
;
Lin MU
;
Wei ZHENG
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Macrophage migration inhibitory factor;
Estradiol
- From:
Chinese Journal of Pathophysiology
2014;33(4):715-718
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of 17β-estradiol on the expression of macrophage migration inhibi-tory factor ( MIF) in cultured endometrial stromal cells from endometriosis .METHODS:Immunohistochemistry was used to identify the endometrial stromal cells .The expression of MIF at mRNA and protein levels was assessed by RT-PCR and Western blotting .RESULTS:Elevated expression of MIF at mRNA and protein levels was observed in the cultured endom-etrial stromal cells treated with 17β-estradiol.In endometrial stromal cells from the women with endometriosis , the level of MIF up-regulation by 17β-estradiol was significantly higher than that in the cells from the women without endometriosis . CONCLUSION:Endometrial stromal cells from endometriosis are more sensitive to 17β-estradiol, which up-regulates the expression of MIF and contribute to the pathogenesis and progression of endometriosis .
