Correlation of Epstein-Barr virus infection with the expression of miR-101, EZH2, and COX-2 in gastric cancer
10.3969/j.issn.1000-8179.20131988
- VernacularTitle:胃癌组织EB病毒感染与miR-101 EZH2 COX-2表达关系的研究
- Author:
Hao YUAN
;
Wei LIU
;
Nan JIANG
;
Yuping WANG
;
Zhaofeng CHEN
;
Min LIU
;
Yongning ZHOU
- Publication Type:Journal Article
- Keywords:
in situ hybridization;
gastric cancer;
miR-101
- From:
Chinese Journal of Clinical Oncology
2014;(7):420-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study was conducted to investigate Epstein-Barr virus (EBV) infection in gastric cancer in Wuwei ar-ea of Gansu province (China) and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas (EBVaGC). Meth-ods:Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-en-coded small RNAs (EBERs), miR-101, EZH2, and COX-2, in gastric cancer tissues (n=120) and the corresponding adjacent tissues (n=120). Results:The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric can-cer (EBVnGC) tissues were significant (P<0.05). The infection of EBV in gastric cancer tissues was positively related to miR-101 ex-pression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues (P<0.05). Conclusion:EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.
